pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11
(Plasmid #174645)

• Purpose: Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes
• Depositing Lab: Lukas Kapitein
• Publication: Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149.

Backbone

• Vector backbone: B-actin
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11
• Species: Synthetic; Physcomitrella patens (spreading earthmoss)
• Insert Size (bp): 4820
• Mutation: SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin14VIb(aa861-1321); mCherry: Met1Del; RAB11: Met1Del
• Promoter: Chicken beta-actin
• Tags / Fusion Proteins:
  • SSPB(micro)-Venus (N terminal on insert)
  • iLID-mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • sequence map from depositor

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Internal plasmid ID: WN480. Phytozome gene ID: Pp3c_4530/8766