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pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro)
(Plasmid #174630)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174630 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    B-actin
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KIF1A(1-365)-VVD52C-GFP-SSPB(micro)
  • Species
    M. musculus (mouse), Synthetic
  • Insert Size (bp)
    2774
  • Mutation
    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile52Cys; EGFP: Met1Del; SSPB:Arg73Gln
  • Entrez Gene
    Kif1a (a.k.a. A, ATSV, C630002N23Rik, Gm1626, Kn, Kns1)
  • Promoter Chicken beta-actin
  • Tag / Fusion Protein
    • GFP-SSPB(micro) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Internal plasmid ID: WN235

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB80-KIF1A(1-365)-VVD52C-GFP-SSPB(micro) was a gift from Lukas Kapitein (Addgene plasmid # 174630 ; http://n2t.net/addgene:174630 ; RRID:Addgene_174630)

  • For your References section:

    An optimized toolbox for the optogenetic control of intracellular transport. Nijenhuis W, van Grinsven MMP, Kapitein LC. J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149. 10.1083/jcb.201907149 PubMed 32328628
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