pB80-iLID-mCherry-RAB11
(Plasmid
#174623)
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PurposeOptogenetic coupling to recycling endosomes (RAB11) via iLID
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174623 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneB-actin
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameiLID-mCherry-RAB11
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)1991
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MutationmCherry: Met1Del; RAB11: Met1Del
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Entrez GeneRAB11A (a.k.a. YL8)
- Promoter Chicken beta-actin
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Tag
/ Fusion Protein
- iLID-mCherry (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Internal plasmid ID: WN105
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB80-iLID-mCherry-RAB11 was a gift from Lukas Kapitein (Addgene plasmid # 174623 ; http://n2t.net/addgene:174623 ; RRID:Addgene_174623) -
For your References section:
An optimized toolbox for the optogenetic control of intracellular transport. Nijenhuis W, van Grinsven MMP, Kapitein LC. J Cell Biol. 2020 Apr 6;219(4). pii: 133834. doi: 10.1083/jcb.201907149. 10.1083/jcb.201907149 PubMed 32328628