PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla
(Plasmid
#174620)
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PurposePiggyBac plasmid expressing EGFP under the control of a cone arrestin promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174620 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePiggyBac
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Backbone manufacturerThe piggyBac vector backbone was a gift from George Church, Addgene plasmid #63800
- Total vector size (bp) 8566
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Vector typeMammalian Expression ; PiggyBac
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameenhanced green fluorescent protein
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Alt nameEGFP
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SpeciesA. victoria
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Insert Size (bp)720
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla was a gift from Volker Busskamp (Addgene plasmid # 174620 ; http://n2t.net/addgene:174620 ; RRID:Addgene_174620) -
For your References section:
Transplanted human cones incorporate and function in a murine cone degeneration model. Gasparini SJ, Tessmer K, Reh M, Wieneke S, Carido M, Volkner M, Borsch O, Swiersy A, Zuzic M, Goureau O, Kurth T, Busskamp V, Zeck G, Karl MO, Ader M. J Clin Invest. 2022 Apr 28. pii: 154619. doi: 10.1172/JCI154619. 10.1172/JCI154619 PubMed 35482419