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Addgene

PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla
(Plasmid #174620)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174620 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    PiggyBac
  • Backbone manufacturer
    The piggyBac vector backbone was a gift from George Church, Addgene plasmid #63800
  • Total vector size (bp) 8566
  • Vector type
    Mammalian Expression ; PiggyBac
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    enhanced green fluorescent protein
  • Alt name
    EGFP
  • Species
    A. victoria
  • Insert Size (bp)
    720

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-mCar-EGFP-WPRE-BGH-pA-UBC-Bla was a gift from Volker Busskamp (Addgene plasmid # 174620 ; http://n2t.net/addgene:174620 ; RRID:Addgene_174620)
  • For your References section:

    Transplanted human cones incorporate and function in a murine cone degeneration model. Gasparini SJ, Tessmer K, Reh M, Wieneke S, Carido M, Volkner M, Borsch O, Swiersy A, Zuzic M, Goureau O, Kurth T, Busskamp V, Zeck G, Karl MO, Ader M. J Clin Invest. 2022 Apr 28. pii: 154619. doi: 10.1172/JCI154619. 10.1172/JCI154619 PubMed 35482419