pETSXM2-pLacI-mtrCAB
(Plasmid #174618)

• Purpose: Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.
• Depositing Lab: I-Son Ng
• Publication: I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Backbone

• Vector backbone: pET-28a
• Backbone size w/o insert (bp): 3903
• Total vector size (bp): 9060
• Modifications to backbone: With pLacI for constitutive expression and RepB replication origin for Shewanella species.
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mtrCAB
• Species: Synthetic; Shewanella oneidensis MR-1
• Insert Size (bp): 3903
• Promoter: pLacI

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (unknown if destroyed)
• 3′ cloning site: NdeI (unknown if destroyed)
• 5′ sequencing primer: AAGAGCTCATGATGAACGCAAAAAAATCAAAAATCGCACTGCTGC
• 3′ sequencing primer: tacatatgttagagcttgtagctcatactcagcattagc

Resource Information

• Supplemental Documents:
  • pETSXM2-pLacI-mtrCAB.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note MtrC starts at amino acid 75. Depositor confirms this is not a concern.