pETSXM2-pLacI-GFP
(Plasmid #174617)

• Purpose: Bacterial expression of GFP.
• Depositing Lab: I-Son Ng
• Publication: I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Backbone

• Vector backbone: pET-28a
• Backbone size w/o insert (bp): 3386
• Total vector size (bp): 4626
• Modifications to backbone: With pLacI for constitutive expression and RepB replication origin for Shewanella species.
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP
• Species: Synthetic
• Insert Size (bp): 1236
• Promoter: pLacI

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: PstI (unknown if destroyed)
• 5′ sequencing primer: TTCTCGAGTGCCACCTGACGTCTAAGAAACC
• 3′ sequencing primer: GGCTGCAGCGGCCGCTACTAGTATATAAACGCAG

Resource Information

• Supplemental Documents:
  • pETSXM2-pLacI-GFP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry