iBE3 CLYBL
(Plasmid #174569)

• Purpose: doxycycline inducible BE3 expression for human CLYBL locus knock-in
• Depositing Lab: Mathew Garnett
• Publication: Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e6. doi: 10.1016/j.ccell.2022.12.009. Epub 2023 Jan 19.

Backbone

• Vector backbone: Addgene plasmid #124229
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin ; mApple

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BE3
• Species: Synthetic
• Promoter: tet ON

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AGCTCGTTTAGTGAACCGTCAGATC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Synthesised gene fragment insert using this paper as a reference https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-018-0617-1. The backbone was from this Addgene plasmid #124229.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2022.03.29.486051 for bioRxiv preprint.