S330
(Plasmid
#174553)
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PurposepET-iBcl2-v4, His tag for affinity purification
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 174553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET
- Backbone size w/o insert (bp) 600
- Total vector size (bp) 6000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameibcl2-v4, bcl2 inhibitor resistant protein
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SpeciesH. sapiens (human)
- Promoter T7
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Tag
/ Fusion Protein
- His
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
S330 was a gift from Bruno Correia (Addgene plasmid # 174553 ; http://n2t.net/addgene:174553 ; RRID:Addgene_174553) -
For your References section:
A rational blueprint for the design of chemically-controlled protein switches. Shui S, Gainza P, Scheller L, Yang C, Kurumida Y, Rosset S, Georgeon S, Di Roberto RB, Castellanos-Rueda R, Reddy ST, Correia BE. Nat Commun. 2021 Oct 1;12(1):5754. doi: 10.1038/s41467-021-25735-9. 10.1038/s41467-021-25735-9 PubMed 34599176