pL106P-LUC-ENTR1A(w451-1)
(Plasmid #17455)

• Purpose: Gateway entry vector, Degron N-term Luciferase
• Depositing Labs: Eric Campeau, Paul Kaufman
• Publication: Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Backbone

• Vector backbone: pENTR1A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2280
• Vector type: Luciferase ; Entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: TOP10F', 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FKBP-Luciferase fusion
• Alt name: L106P-Luciferase fusion
• Species: firefly
• Insert Size (bp): 1982
• GenBank ID: AAA89088
• Entrez Gene: MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
• Tag / Fusion Protein:
  • FKBP12DD (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: ENTRforw
• 3′ sequencing primer: ENTRrev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: L106P-LUC was generated in the Wandless lab (pBMN FKBP L106P-PpLuc IRES Blasticidin plasmid)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry