pLKO.1 mito-TagRFP-T
(Plasmid
#174543)
-
Purposelabels mitochondria with TagRFP-T
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 174543 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepLKO.1
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameMTS-tagRFP-T
-
SpeciesH. sapiens (human)
-
Tag
/ Fusion Protein
- tagRFP-T (C terminal on insert)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.08.10.455713 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pLKO.1 mito-TagRFP-T was a gift from Minna Roh-Johnson (Addgene plasmid # 174543 ; http://n2t.net/addgene:174543 ; RRID:Addgene_174543) -
For your References section:
Transferred mitochondria accumulate reactive oxygen species, promoting proliferation. Kidwell CU, Casalini JR, Pradeep S, Scherer SD, Greiner D, Bayik D, Watson DC, Olson GS, Lathia JD, Johnson JS, Rutter J, Welm AL, Zangle TA, Roh-Johnson M. Elife. 2023 Mar 6;12:e85494. doi: 10.7554/eLife.85494. 10.7554/eLife.85494 PubMed 36876914