EF1a-mito-dsRED2
(Plasmid
#174541)
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Purposemitochondria targeted dsRED expressed off of EF1a promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174541 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLVX
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Backbone manufacturerClonetech
- Backbone size w/o insert (bp) 8378
- Total vector size (bp) 9164
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMito
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Alt nameCOX8A presequence
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SpeciesH. sapiens (human)
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Insert Size (bp)72
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GenBank IDNM_004074.3 NM_004074.3
- Promoter EF1a
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Tag
/ Fusion Protein
- dsRED (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymito-dsRED was cloned from addgene plasmid 55838.
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EF1a-mito-dsRED2 was a gift from Thomas Schwarz (Addgene plasmid # 174541 ; http://n2t.net/addgene:174541 ; RRID:Addgene_174541)
