pKSB- attP 3xP3-GFPsv40 GGGG AAGA
(Plasmid
#174534)
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PurposeattP site and 3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoes
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174534 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepKSB-
- Backbone size w/o insert (bp) 2961
- Total vector size (bp) 4200
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameattP and 3xP3-GFP-SV40 BsaI cassette
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SpeciesSynthetic
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Insert Size (bp)1300
- Promoter 3xP3
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
attP site within the fluorescence marker cassette is to allow subsequent insertions of attB plasmids in the knock-in locus
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKSB- attP 3xP3-GFPsv40 GGGG AAGA was a gift from Eric Marois (Addgene plasmid # 174534 ; http://n2t.net/addgene:174534 ; RRID:Addgene_174534)
