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4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)
(Plasmid #174529)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174529 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB53
  • Total vector size (bp) 10600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    PylS
  • Alt name
    MmPylRS
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    1395
  • GenBank ID
    NC_003901.1
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    PylT
  • Species
    Methanosarcina mazei
  • Insert Size (bp)
    1400
  • Promoter U6

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA) was a gift from Yu-Hsuan Tsai (Addgene plasmid # 174529 ; http://n2t.net/addgene:174529 ; RRID:Addgene_174529)

  • For your References section:

    Development of mammalian cell logic gates controlled by unnatural amino acids. Mills EM, Barlow VL, Jones AT, Tsai YH. Cell Rep Methods. 2021 Sep 16;1(6):100073. doi: 10.1016/j.crmeth.2021.100073. eCollection 2021 Oct 25. 10.1016/j.crmeth.2021.100073 PubMed 35474893
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