pRS426-HsFECH
(Plasmid #174524)

• Purpose: Expresses human ferrochelatase (FECH) in yeast
• Depositing Lab: Oleh Khalimonchuk
• Publication: Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.1016/j.redox.2021.102125. Epub 2021 Sep 10.

Backbone

• Vector backbone: pRS426
• Backbone size w/o insert (bp): 5726
• Total vector size (bp): 7306
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FECH with yeast HEM15 promoter and terminator
• Alt name: EPP1
• Species: H. sapiens (human), S. cerevisiae (budding yeast), Synthetic
• Insert Size (bp): 1460
• Mutation: Lacks original mitochondrial targeting sequence (162 bp), which has been replaced with a 350 bp fragment that contains the S. cerevisiae ferrochelatase Hem15 promoter and targeting sequence.
• GenBank ID: 2235
• Entrez Gene: FECH (a.k.a. EPP, EPP1, FCE)
• Promoter: HEM15 (yeast, 257 bp)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: M13 forward
• 3′ sequencing primer: M13 reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This construct was subcloned from the original pRS316-Hem15/FECH plasmid generated by Harry Dailey lab at UGA. See Medlock et al. Biochemistry 2012, 51, 27, 5422–5433. Obtained from Amy Medlock/Harry Dailey

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: The plasmid contain 17 nucleotide discrepancies at N-terminus of the FEECH sequence compared to the depositor's provided sequence. The depositor noted that these differences do not affect the plasmid's function.