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pRS426-CeLACE-1
(Plasmid #174523)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174523 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS46
  • Backbone size w/o insert (bp) 6338
  • Total vector size (bp) 10376
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lace-1
  • Alt name
    C30F12.2
  • Alt name
    AFG1L
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1338
  • Entrez Gene
    C30F12.2 (a.k.a. CELE_C30F12.2)
  • Promoter MET25
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer M13 (universal) forward
  • 3′ sequencing primer M13 (universal) reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS426-CeLACE-1 was a gift from Oleh Khalimonchuk (Addgene plasmid # 174523 ; http://n2t.net/addgene:174523 ; RRID:Addgene_174523)

  • For your References section:

    The AAA ATPase Afg1 preserves mitochondrial fidelity and cellular health by maintaining mitochondrial matrix proteostasis. Germany EM, Zahayko N, Huebsch ML, Fox JL, Prahlad V, Khalimonchuk O. J Cell Sci. 2018 Nov 21;131(22). pii: jcs.219956. doi: 10.1242/jcs.219956. 10.1242/jcs.219956 PubMed 30301782
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