pCDNA3-mitoT
(Plasmid #174519)

• Purpose: Engineered intermembrane bridge to link the inner and outer mitochondrial membranes in mammalian cells
• Depositing Lab: Oleh Khalimonchuk
• Publication: Viana et al iScience. 2021 Jan 29;24(2):102119. doi: 10.1016/j.isci.2021.102119. eCollection 2021 Feb 19.

Backbone

• Vector backbone: pcDNA3.1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MitoT synthetic tether
• Alt name: MitoT
• Species: H. sapiens (human), Synthetic; E.coli
• Insert Size (bp): 468
• Promoter: CMV
• Tags / Fusion Proteins:
  • 6xHis (C terminal on insert)
  • eGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: 5’-AAAGGATCCATGGCGATGCTGGTCCTAGTACCC-3’
• 3′ sequencing primer: 5’-CAAGAATTCGTGATGGTGATGGTGATGGAAGTAGATGCAGTACCCA-3’

Resource Information

• Supplemental Documents:
  • pCDNA3-mitoT support file 1 (full sequence)
  • pCDNA3-mitoT support file 2 (insert sequence)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry