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pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)
(Plasmid #174516)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174516 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMB1
  • Backbone size w/o insert (bp) 3068
  • Total vector size (bp) 5055
  • Modifications to backbone
    The whole backbone was recoded according to Syn61
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    PylS_1R26 - CBZK
  • Insert Size (bp)
    838
  • Mutation
    Recoded; Y126G-M129L
  • Promoter GlnS

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Af_TyrRS - p-I-Phe
  • Species
    A.fulgidus
  • Insert Size (bp)
    972
  • Mutation
    Recoded; Y36I-L69M-H74L-Q116E-D165T-I166G
  • Promoter GlnS

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    Ma tRNA pylT(8) with CGA anticodon
  • Species
    M. alvus
  • Insert Size (bp)
    72
  • Mutation
    M.alvus Pyl tRNA with CGA anticodon
  • Promoter lpp

Cloning Information for Gene/Insert 3

Gene/Insert 4

  • Gene/Insert name
    A.fulgidus tRNA with CUA anticodon
  • Species
    A.fulgidus
  • Mutation
    CUA anticodon
  • Promoter lpp

Cloning Information for Gene/Insert 4

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA) was a gift from Jason W Chin (Addgene plasmid # 174516 ; http://n2t.net/addgene:174516 ; RRID:Addgene_174516)

  • For your References section:

    Sense codon reassignment enables viral resistance and encoded polymer synthesis. Robertson WE, Funke LFH, de la Torre D, Fredens J, Elliott TS, Spinck M, Christova Y, Cervettini D, Boge FL, Liu KC, Buse S, Maslen S, Salmond GPC, Chin JW. Science. 2021 Jun 4;372(6546):1057-1062. doi: 10.1126/science.abg3029. 10.1126/science.abg3029 PubMed 34083482
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