pCS2+-wbp2nlF127P
(Plasmid
#174507)
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Purposeexpresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174507 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4100
- Total vector size (bp) 5045
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Vector typeXenopus Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namewbp2nlF127P
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SpeciesX. laevis (frog)
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Insert Size (bp)948
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Mutationchanged Phenylalanine at aa127 to Proline
- Promoter SP6
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Tag
/ Fusion Protein
- none
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Cla1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
we cloned this plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+-wbp2nlF127P was a gift from Sally Moody (Addgene plasmid # 174507 ; http://n2t.net/addgene:174507 ; RRID:Addgene_174507) -
For your References section:
Wbp2nl has a developmental role in establishing neural and non-neural ectodermal fates. Marchak A, Grant PA, Neilson KM, Datta Majumdar H, Yaklichkin S, Johnson D, Moody SA. Dev Biol. 2017 Sep 1;429(1):213-224. doi: 10.1016/j.ydbio.2017.06.025. Epub 2017 Jun 27. 10.1016/j.ydbio.2017.06.025 PubMed 28663133