Pxyl-ftsZ-sfGFP
(Plasmid
#174505)
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PurposeIntegrated plasmid harboring ftsZ-sfGFP gene, inducible by xylose (sequence of sfGFP optimized)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174505 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepXGFPC2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameftsZ
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SpeciesC. crescentus
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Tag
/ Fusion Protein
- superfold GFP (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made bysfGFP gene was synthesized by the Twist Bioscience company
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pxyl-ftsZ-sfGFP was a gift from Suliana Manley (Addgene plasmid # 174505 ; http://n2t.net/addgene:174505 ; RRID:Addgene_174505) -
For your References section:
Event-driven acquisition for content-enriched microscopy. Mahecic D, Stepp WL, Zhang C, Griffie J, Weigert M, Manley S. Nat Methods. 2022 Sep 8. pii: 10.1038/s41592-022-01589-x. doi: 10.1038/s41592-022-01589-x. 10.1038/s41592-022-01589-x PubMed 36076039