pnCS_SEPT7_SEPT9_i5-TEV-Strep
(Plasmid #174502)

• Purpose: bacterial co-expression of human SEPT7 and of human SEPT9_i5
• Depositing Lab: Manos Mavrakis
• Publication: Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484.

Backbone

• Vector backbone: pnCS
• Total vector size (bp): 5581
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: SEPTIN7
• Alt name: SEPT7
• Species: H. sapiens (human)
• Entrez Gene: SEPTIN7 (a.k.a. CDC10, CDC3, NBLA02942, SEPT7, SEPT7A)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: SEPTIN9
• Alt name: SEPT9
• Alt name: SEPT9 isoform 5
• Species: H. sapiens (human)
• Entrez Gene: SEPTIN9 (a.k.a. AF17q25, MSF, MSF1, NAPB, PNUTL4, SEPT9, SINT1, SeptD1)
• Tag / Fusion Protein:
  • TEV-Strep (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pnCS_SEPT7_SEPT9_i5-TEV-Strep.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

SEPT9 isoform 5 is the protein product of transcript variant 7 (NCBI Reference Sequence: NP_001106968.1).