pFLAG-HHARI
(Plasmid #17450)

• Depositing Lab: Dong-Er Zhang
• Publication: Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Backbone

• Vector backbone: pFlag-CMV2
• Backbone manufacturer: Sigma
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HHARI
• Species: H. sapiens (human)
• Entrez Gene: ARIH1 (a.k.a. ARI, HARI, HHARI, UBCH7BP)
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Step1: HindIII-human HHARI (about 700bp, 5’ portion)-EcoRV fragment was made by PCR. Step2: Digestion of pCMV-sport6-HHAR1 with EcoR V + Not I, producing 5.3Kb, 1.2Kb fragments, take back 1.2 Kb fragment. Step3: Digestion of pFlagCMV2 with Hind III + Not I, taking back the vector 5.4 Kb. Step4: Ligation of 3 fragments.