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Addgene

pnCS_SEPT6_SEPT7-TEV-Strep
(Plasmid #174499)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174499 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pnCS
  • Total vector size (bp) 6169
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SEPTIN6
  • Alt name
    SEPT6
  • Species
    H. sapiens (human)
  • Entrez Gene
    SEPTIN6 (a.k.a. SEP2, SEPT2, SEPT6)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    SEPTIN7
  • Alt name
    SEPT7
  • Species
    H. sapiens (human)
  • Entrez Gene
    SEPTIN7 (a.k.a. CDC10, CDC3, NBLA02942, SEPT7, SEPT7A)
  • Tag / Fusion Protein
    • TEV-Strep (C terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pnCS_SEPT6_SEPT7-TEV-Strep was a gift from Manos Mavrakis (Addgene plasmid # 174499 ; http://n2t.net/addgene:174499 ; RRID:Addgene_174499)
  • For your References section:

    Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms. Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, Cabantous S, Koenderink GH, Bertin A, Mavrakis M. J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484. 10.1242/jcs.258484 PubMed 34237139