pnEA-vH_His-TEV-SEPT2_SEPT6
(Plasmid #174497)

• Purpose: bacterial co-expression of human SEPT2 and of human SEPT6
• Depositing Lab: Manos Mavrakis
• Publication: Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484.

Backbone

• Vector backbone: pnEA-vH
• Total vector size (bp): 8169
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: SEPTIN2
• Alt name: SEPT2
• Species: H. sapiens (human)
• Entrez Gene: SEPTIN2 (a.k.a. DIFF6, NEDD-5, NEDD5, Pnutl3, SEPT2, hNedd5)
• Tag / Fusion Protein:
  • His6-TEV (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: SEPTIN6
• Alt name: SEPT6
• Species: H. sapiens (human)
• Entrez Gene: SEPTIN6 (a.k.a. SEP2, SEPT2, SEPT6)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pnEA-vH_His-TEV-SEPT2_SEPT6.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry