pnCS_mEGFP-DSep2_Peanut-Strep
(Plasmid #174496)

• Purpose: bacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila Peanut
• Depositing Lab: Manos Mavrakis
• Publication: Mavrakis et al Methods Cell Biol. 2016;136:199-220. doi: 10.1016/bs.mcb.2016.03.020. Epub 2016 Jun 14.

Backbone

• Vector backbone: pnCS
• Total vector size (bp): 7149
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Sep2
• Alt name: DSep2
• Alt name: Septin 2
• Species: D. melanogaster (fly)
• Entrez Gene: Sep2 (a.k.a. Dmel_CG4173, 38961, CG4173, DSep2, Dmel\CG4173, dSEPT2, sep2)
• Tag / Fusion Protein:
  • mEGFP (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: peanut
• Species: D. melanogaster (fly)
• Entrez Gene: pnut (a.k.a. Dmel_CG8705, CG8705, Dmel\CG8705, E(sina)44C, E(sina)8, PNUT, Peanut, Pnut, anon-EST:Liang-2.18, clone 2.18, dSEPT7, l(2)02502)
• Tag / Fusion Protein:
  • Strep (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pnCS_mEGFP-DSep2_Peanut-Strep.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For protein expression, grow at 17degC.

Plasmid used in the following publication:

Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms.
Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, Cabantous S, Koenderink GH, Bertin A, Mavrakis M
J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484.