pnEA-vH_His-TEV-DSep1
(Plasmid #174493)

• Purpose: bacterial expression of Drosophila Sep1
• Depositing Lab: Manos Mavrakis
• Publication: Mavrakis et al Nat Cell Biol. 2014 Apr;16(4):322-34. doi: 10.1038/ncb2921. Epub 2014 Mar 16.

Backbone

• Vector backbone: pnEA-vH
• Total vector size (bp): 6831
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sep1
• Alt name: Septin 1
• Species: D. melanogaster (fly)
• Entrez Gene: Sep1 (a.k.a. Dmel_CG1403, 38960, CG1403, DSep1, Diff6, Dmel\CG1403, anon-19Fa, dSEPT1, iby, sep1, tu1)
• Tag / Fusion Protein:
  • His6-TEV (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminal

Resource Information

• Supplemental Documents:
  • pnEA-vH_His-TEV-DSep1.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid used in the following publications:

Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms.
Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, Cabantous S, Koenderink GH, Bertin A, Mavrakis M
J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484.

Purification of recombinant human and Drosophila septin hexamers for TIRF assays of actin-septin filament assembly.
M Mavrakis, F-C Tsai, G H Koenderink
Methods Cell Biol
2016;136:199-220. doi: 10.1016/bs.mcb.2016.03.020. Epub 2016 Jun 14. PMID: 27473911 DOI: 10.1016/bs.mcb.2016.03.020