pnEA-vH_His-TEV-SEPT2-msfGFP
(Plasmid
#174492)
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Purposebacterial expression of human SEPT2 fused to monomeric superfolder GFP
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174492 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepnEA-vH
- Total vector size (bp) 7545
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSEPTIN2
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Alt nameSEPT2
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SpeciesH. sapiens (human)
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Entrez GeneSEPTIN2 (a.k.a. DIFF6, NEDD-5, NEDD5, Pnutl3, SEPT2, hNedd5)
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Tags
/ Fusion Proteins
- His6-TEV (N terminal on insert)
- msfGFP (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For protein expression, grow at 17C.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pnEA-vH_His-TEV-SEPT2-msfGFP was a gift from Manos Mavrakis (Addgene plasmid # 174492 ; http://n2t.net/addgene:174492 ; RRID:Addgene_174492) -
For your References section:
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms. Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, Cabantous S, Koenderink GH, Bertin A, Mavrakis M. J Cell Sci. 2021 Jul 8. pii: 270842. doi: 10.1242/jcs.258484. 10.1242/jcs.258484 PubMed 34237139
