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Addgene

pSNRW-Z5-Nluc
(Plasmid #174487)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174487 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5292
  • Total vector size (bp) 6700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Z5-Nluc
  • Species
    Staphylococcus aureus, Oplophorus gracilirostris
  • Insert Size (bp)
    1404
  • GenBank ID
    AFI79290.1 41071997
  • Promoter T7lac promoter
  • Tag / Fusion Protein
    • 6x Histidine tag (N terminal on insert) (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGCGGCAGCCATATG
  • 3′ sequencing primer GTGGTGGTGGTGGTGC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSNRW-Z5-Nluc was a gift from Richard C. Willson (Addgene plasmid # 174487 ; http://n2t.net/addgene:174487 ; RRID:Addgene_174487)

  • For your References section:

    Protein A-nanoluciferase fusion protein for generalized, sensitive detection of immunoglobulin G. Nandy S, Crum M, Wasden K, Strych U, Goyal A, Maranholkar V, Mo W, Vu B, Kourentzi K, Willson RC. Anal Biochem. 2022 Oct 18:114929. doi: 10.1016/j.ab.2022.114929. 10.1016/j.ab.2022.114929 PubMed 36270332
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