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Addgene

pFX-Lifeact-SECFP
(Plasmid #174476)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174476 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFX
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4830
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SECFP with actin-targeting sequence
  • Insert Size (bp)
    830
  • Promoter CMV
  • Tag / Fusion Protein
    • Lifeact (N-terminal 17 amino acids from S. Cerevisiae Abp140) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFX-Lifeact-SECFP was a gift from Yusuke Ohba (Addgene plasmid # 174476 ; http://n2t.net/addgene:174476 ; RRID:Addgene_174476)

  • For your References section:

    Folding Latency of Fluorescent Proteins Affects the Mitochondrial Localization of Fusion Proteins. Kashiwagi S, Fujioka Y, Satoh AO, Yoshida A, Fujioka M, Nepal P, Tsuzuki A, Aoki O, Paudel S, Sasajima H, Ohba Y. Cell Struct Funct. 2019 Dec 26;44(2):183-194. doi: 10.1247/csf.19028. Epub 2019 Nov 15. 10.1247/csf.19028 PubMed 31735740
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