pFX-Venus-Sec61β
(Plasmid #174467)

• Purpose: Express Venus with endoplasmic reticulum-targeting sequence in mammalian cells
• Depositing Lab: Yusuke Ohba
• Publication: Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183-194. doi: 10.1247/csf.19028. Epub 2019 Nov 15.

Backbone

• Vector backbone: pFX
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 5000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Venus with endoplasmic reticulum-targeting sequence
• Insert Size (bp): 1000
• Promoter: CMV
• Tag / Fusion Protein:
  • Sec61 subunit beta (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: none
• 3′ sequencing primer: none

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry