pIVT-EGFP-mouseCENP-C
(Plasmid
#174444)
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Purposein vitro transcription template for mouse CENP-C (N-ter EGFP tagged)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174444 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepIVT
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Vector typein vitro transcription template
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCentromere protein C
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Alt nameCENP-C
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SpeciesM. musculus (mouse)
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Insert Size (bp)2721
- Promoter T7 SP6
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CAGGAAACAGCTATGAC
- 3′ sequencing primer GTAAAACGACGGCCAGT (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pIVT-EGFP-mouseCENP-C was a gift from Michael Lampson (Addgene plasmid # 174444 ; http://n2t.net/addgene:174444 ; RRID:Addgene_174444) -
For your References section:
Parallel pathways for recruiting effector proteins determine centromere drive and suppression. Kumon T, Ma J, Akins RB, Stefanik D, Nordgren CE, Kim J, Levine MT, Lampson MA. Cell. 2021 Sep 16;184(19):4904-4918.e11. doi: 10.1016/j.cell.2021.07.037. Epub 2021 Aug 24. 10.1016/j.cell.2021.07.037 PubMed 34433012