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Addgene

pIVT-dCas9-EGFP
(Plasmid #174442)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174442 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIVT
  • Vector type
    in vitro transcription template

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cas9 Endonuclease Dead
  • Alt name
    dCas9
  • Species
    Streptococcus pyogenes
  • Insert Size (bp)
    4104
  • Promoter T7 SP6
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CAGGAAACAGCTATGAC
  • 3′ sequencing primer GTAAAACGACGGCCAGT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIVT-dCas9-EGFP was a gift from Michael Lampson (Addgene plasmid # 174442 ; http://n2t.net/addgene:174442 ; RRID:Addgene_174442)

  • For your References section:

    Parallel pathways for recruiting effector proteins determine centromere drive and suppression. Kumon T, Ma J, Akins RB, Stefanik D, Nordgren CE, Kim J, Levine MT, Lampson MA. Cell. 2021 Sep 16;184(19):4904-4918.e11. doi: 10.1016/j.cell.2021.07.037. Epub 2021 Aug 24. 10.1016/j.cell.2021.07.037 PubMed 34433012
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