pcDNA3-pmEMBer
(Plasmid #174441)

• Purpose: Plasma membrane-targeted ERK monobody binder for local inhibition of ERK activity in live cells.
• Depositing Lab: Jin Zhang
• Publication: Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 10.7554/eLife.57410.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5472
• Total vector size (bp): 6576
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pmEMBer
• Alt name: EMBer7.1-mCherry-Kras
• Insert Size (bp): 1104
• Promoter: CMV
• Tags / Fusion Proteins:
  • 6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
  • C-terminal targeting sequence from Kras (C terminal on insert)
  • Myc epitope tag (C terminal on insert)
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3-EMBer7.1-mCherry-Kras.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry