Skip to main content
Addgene

pEGFP-hsp27 wt FL
(Plasmid #17444)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17444 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEGFP-C2
  • Backbone manufacturer
    BD Biosciences Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Growth instructions
    TOP-10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    small heat shock protein27
  • Alt name
    HSPB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    633
  • GenBank ID
    BC012768
  • Entrez Gene
    HSPB1 (a.k.a. CMT2F, HEL-S-102, HMN2B, HMND3, HS.76067, HSP27, HSP28, Hsp25, SRP27)
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR I (not destroyed)
  • 3′ cloning site Sal I (not destroyed)
  • 5′ sequencing primer GGGATCCTAGAATTCGCCCACCATGACCGAGCGCCGCGT
  • 3′ sequencing primer CTCGAGGTCGACCTTGGCGGCAGTCTCATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-hsp27 wt FL was a gift from Andrea Doseff (Addgene plasmid # 17444 ; http://n2t.net/addgene:17444 ; RRID:Addgene_17444)

  • For your References section:

    Binding of caspase-3 prodomain to heat shock protein 27 regulates monocyte apoptosis by inhibiting caspase-3 proteolytic activation. Voss OH, Batra S, Kolattukudy SJ, Gonzalez-Mejia ME, Smith JB, Doseff AI. J Biol Chem. 2007 Aug 24. 282(34):25088-99. 10.1074/jbc.M701740200 PubMed 17597071
Related items:
Commonly requested with: