Drp1-StrepII
(Plasmid
#174428)
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PurposeExpresses human Drp1 Isoform 3 in bacteria
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174428 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET15b
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDrp1
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SpeciesH. sapiens (human)
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MutationNone
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GenBank IDNM_005690.5
- Promoter T7
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Tag
/ Fusion Protein
- StrepII (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7 forward
- 3′ sequencing primer T7 reverse (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Drp1-StrepII was a gift from Thomas Pucadyil (Addgene plasmid # 174428 ; http://n2t.net/addgene:174428 ; RRID:Addgene_174428) -
For your References section:
Dynamin-related protein 1 has membrane constricting and severing abilities sufficient for mitochondrial and peroxisomal fission. Kamerkar SC, Kraus F, Sharpe AJ, Pucadyil TJ, Ryan MT. Nat Commun. 2018 Dec 7;9(1):5239. doi: 10.1038/s41467-018-07543-w. 10.1038/s41467-018-07543-w PubMed 30531964
