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Addgene

6xHis(TEV)-Drp1(K569A,K571A)-StrepII
(Plasmid #174426)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174426 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Drp1
  • Species
    H. sapiens (human)
  • Mutation
    K569A, K571A
  • GenBank ID
    NM_005690.5
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis(TEV) (N terminal on insert)
    • StrepII (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    6xHis(TEV)-Drp1(K569A,K571A)-StrepII was a gift from Thomas Pucadyil (Addgene plasmid # 174426 ; http://n2t.net/addgene:174426 ; RRID:Addgene_174426)

  • For your References section:

    Dynamin-related protein 1 has membrane constricting and severing abilities sufficient for mitochondrial and peroxisomal fission. Kamerkar SC, Kraus F, Sharpe AJ, Pucadyil TJ, Ryan MT. Nat Commun. 2018 Dec 7;9(1):5239. doi: 10.1038/s41467-018-07543-w. 10.1038/s41467-018-07543-w PubMed 30531964
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