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Addgene

MSCV-IRES-Thy1.1 DEST
(Plasmid #17442)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17442 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-IRES-Thy1.1
  • Backbone manufacturer
    Philippa Marrack Lab
  • Backbone size (bp) 6063
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Thy1.1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site attB1 (not destroyed)
  • 3′ cloning site attB2 (not destroyed)
  • 5′ sequencing primer pBABE5'
  • 3′ sequencing primer IRES-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Recombination sites were added to MSCV-IRES-Thy1.1 for subcloning from pENTR vectors.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSCV-IRES-Thy1.1 DEST was a gift from Anjana Rao (Addgene plasmid # 17442 ; http://n2t.net/addgene:17442 ; RRID:Addgene_17442)

  • For your References section:

    FOXP3 controls regulatory T cell function through cooperation with NFAT. Wu Y, Borde M, Heissmeyer V, Feuerer M, Lapan AD, Stroud JC, Bates DL, Guo L, Han A, Ziegler SF, Mathis D, Benoist C, Chen L, Rao A. Cell. 2006 Jul 28. 126(2):375-87. 10.1016/j.cell.2006.05.042 PubMed 16873067
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