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Addgene

pWSK129-stm2585_Y167F
(Plasmid #174402)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174402 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWSK129
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sarA
  • Alt name
    stm2585
  • Alt name
    steE
  • Species
    Salmonella enterica ser. Typhimurium
  • Insert Size (bp)
    1360
  • Mutation
    sarA with Y167F mutation
  • Promoter Endogenous

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site ApaI (not destroyed)
  • 5′ sequencing primer M13-rev
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWSK129-stm2585_Y167F was a gift from Dennis Ko (Addgene plasmid # 174402 ; http://n2t.net/addgene:174402 ; RRID:Addgene_174402)

  • For your References section:

    The Salmonella Secreted Effector SarA/SteE Mimics Cytokine Receptor Signaling to Activate STAT3. Gibbs KD, Washington EJ, Jaslow SL, Bourgeois JS, Foster MW, Guo R, Brennan RG, Ko DC. Cell Host Microbe. 2020 Jan 8;27(1):129-139.e4. doi: 10.1016/j.chom.2019.11.012. Epub 2019 Dec 31. 10.1016/j.chom.2019.11.012 PubMed 31901521
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