pWSK129-stm2585
(Plasmid #174401)

• Purpose: S. Typhimurium stm2585 (sarA/steE) flanked with endogenous regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial expression.
• Depositing Lab: Dennis Ko
• Publication: Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-139.e4. doi: 10.1016/j.chom.2019.11.012. Epub 2019 Dec 31.

Backbone

• Vector backbone: pWSK129
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: sarA
• Alt name: stm2585
• Alt name: steE
• Species: Salmonella enterica ser. Typhimurium
• Insert Size (bp): 1360

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: M13-rev
• 3′ sequencing primer: M13-fwd and T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: From Sarah Jaslow (Jaslow et al. 2018)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry