pcDNA3.1-Flag-gp130dimer-stm2585
(Plasmid
#174395)
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PurposeConstitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174395 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameIL6ST cytosolic domain and sarA chimera
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Alt namegp130-stm2585 chimera
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGenScript
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-Flag-gp130dimer-stm2585 was a gift from Dennis Ko (Addgene plasmid # 174395 ; http://n2t.net/addgene:174395 ; RRID:Addgene_174395) -
For your References section:
The Salmonella Secreted Effector SarA/SteE Mimics Cytokine Receptor Signaling to Activate STAT3. Gibbs KD, Washington EJ, Jaslow SL, Bourgeois JS, Foster MW, Guo R, Brennan RG, Ko DC. Cell Host Microbe. 2020 Jan 8;27(1):129-139.e4. doi: 10.1016/j.chom.2019.11.012. Epub 2019 Dec 31. 10.1016/j.chom.2019.11.012 PubMed 31901521