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Addgene

pcDNA3.1-Flag-stm2585-gp130
(Plasmid #174393)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174393 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sarA-IL6ST chimera
  • Alt name
    stm2585-gp130 chimera
  • Species
    S. enterica ser. Typhimurium
  • Insert Size (bp)
    567
  • Mutation
    140-177 of sarA replaced with homologous IL6ST sequence
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Flag-stm2585-gp130 was a gift from Dennis Ko (Addgene plasmid # 174393 ; http://n2t.net/addgene:174393 ; RRID:Addgene_174393)

  • For your References section:

    The Salmonella Secreted Effector SarA/SteE Mimics Cytokine Receptor Signaling to Activate STAT3. Gibbs KD, Washington EJ, Jaslow SL, Bourgeois JS, Foster MW, Guo R, Brennan RG, Ko DC. Cell Host Microbe. 2020 Jan 8;27(1):129-139.e4. doi: 10.1016/j.chom.2019.11.012. Epub 2019 Dec 31. 10.1016/j.chom.2019.11.012 PubMed 31901521
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