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Addgene

pBM23-pyrF-NT
(Plasmid #174384)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174384 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBM23
  • Vector type
    Bacterial Expression ; Template for cloning fragments for chromosomal integration into Haloferax mediterranei

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pyrF-NT
  • gRNA/shRNA sequence
    mini-CRISPR (NT)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBM23-pyrF-NT was a gift from Jing Han (Addgene plasmid # 174384 ; http://n2t.net/addgene:174384 ; RRID:Addgene_174384)

  • For your References section:

    Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux. Lin L, Chen J, Mitra R, Gao Q, Cheng F, Xu T, Zuo Z, Xiang H, Han J. Commun Biol. 2021 Aug 25;4(1):1007. doi: 10.1038/s42003-021-02541-z. 10.1038/s42003-021-02541-z PubMed 34433872
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