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Addgene

pWL502-crRNA
(Plasmid #174383)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174383 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWL502
  • Backbone size w/o insert (bp) 7876
  • Total vector size (bp) 8018
  • Vector type
    Bacterial Expression, CRISPR ; archaeal expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    a crRNA targeting the template strand
  • gRNA/shRNA sequence
    GTCAGTCGATTTATGTTCTCAACGTCCTTACAAAG
  • Promoter PhaR promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACAACAACCCCCCATGGATCCAATGGTGTCGAAGGGAAC
  • 3′ sequencing primer CGCACACAAGAAAACGGTACCAAAAAAAAGCTTCAACCC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWL502-crRNA was a gift from Jing Han (Addgene plasmid # 174383 ; http://n2t.net/addgene:174383 ; RRID:Addgene_174383)

  • For your References section:

    Optimising PHBV biopolymer production in haloarchaea via CRISPRi-mediated redirection of carbon flux. Lin L, Chen J, Mitra R, Gao Q, Cheng F, Xu T, Zuo Z, Xiang H, Han J. Commun Biol. 2021 Aug 25;4(1):1007. doi: 10.1038/s42003-021-02541-z. 10.1038/s42003-021-02541-z PubMed 34433872
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