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Addgene

pET-pA-Hia5
(Plasmid #174372)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174372 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Total vector size (bp) 6534
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For protein expression, use T7 Express lysY competent E. coli cells (NEB #C3010I) See protocol: dx.doi.org/10.17504/protocols.io.bv82n9ye
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    protein A - hia5
  • Insert Size (bp)
    1500
  • Tags / Fusion Proteins
    • Protein A (N terminal on insert)
    • 6xHis (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositing laboratory recommends Addgene Plasmid 222303: prhaBAD-pA-Hia5, an improved version of this plasmid.
Protocol for purification and expression can be found at: dx.doi.org/10.17504/protocols.io.bv82n9ye
Application for DiMeLo-seq is described in: doi.org/10.1101/2021.07.06.451383
Protocol was adapted and modified from: doi.org/10.1126/science.aaz1646

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-pA-Hia5 was a gift from Aaron Straight (Addgene plasmid # 174372 ; http://n2t.net/addgene:174372 ; RRID:Addgene_174372)

  • For your References section:

    DiMeLo-seq: a long-read, single-molecule method for mapping protein-DNA interactions genome wide. Altemose N, Maslan A, Smith OK, Sundararajan K, Brown RR, Mishra R, Detweiler AM, Neff N, Miga KH, Straight AF, Streets A. Nat Methods. 2022 Jun;19(6):711-723. doi: 10.1038/s41592-022-01475-6. Epub 2022 Apr 8. 10.1038/s41592-022-01475-6 PubMed 35396487
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