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Addgene

C211
(Plasmid #174360)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174360 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PABPC1
  • Species
    H. sapiens (human)
  • Mutation
    I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K and multiple silent mutations introduced to be resistant to siRNAs
  • GenBank ID
    NM_002568
  • Entrez Gene
    PABPC1 (a.k.a. PAB1, PABP, PABP1, PABPC2, PABPL1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C211 was a gift from David Bartel (Addgene plasmid # 174360 ; http://n2t.net/addgene:174360 ; RRID:Addgene_174360)

  • For your References section:

    The molecular basis of coupling between poly(A)-tail length and translational efficiency. Xiang K, Bartel DP. Elife. 2021 Jul 2;10. pii: 66493. doi: 10.7554/eLife.66493. 10.7554/eLife.66493 PubMed 34213414
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