C205
(Plasmid
#174359)
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PurposeFor expressing siRNA-resistant frog ePAB in human cell lines via transfection
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 174359 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameePAB
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SpeciesX. laevis (frog)
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MutationMultiple silent mutations introduced to be resistant to siRNAs
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GenBank IDNM_001091882.1
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
C205 was a gift from David Bartel (Addgene plasmid # 174359 ; http://n2t.net/addgene:174359 ; RRID:Addgene_174359) -
For your References section:
The molecular basis of coupling between poly(A)-tail length and translational efficiency. Xiang K, Bartel DP. Elife. 2021 Jul 2;10. pii: 66493. doi: 10.7554/eLife.66493. 10.7554/eLife.66493 PubMed 34213414