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Addgene

C152
(Plasmid #174355)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 174355 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Unknown

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Auxin-inducible degron (AID) and mCherry

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

AID, T2A and mCherry are flanked by homology arms of human PABPC1 genomic regions near the stop codon. Used with C119 and the PAM region is mutated.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C152 was a gift from David Bartel (Addgene plasmid # 174355 ; http://n2t.net/addgene:174355 ; RRID:Addgene_174355)

  • For your References section:

    The molecular basis of coupling between poly(A)-tail length and translational efficiency. Xiang K, Bartel DP. Elife. 2021 Jul 2;10. pii: 66493. doi: 10.7554/eLife.66493. 10.7554/eLife.66493 PubMed 34213414
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