C064
(Plasmid
#174353)
-
PurposePlasmid for expressing frog PABPC1 (C-His) in E.coli
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 174353 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepET28a
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namePABPC1
-
SpeciesX. laevis (frog)
-
GenBank IDM27072.1
-
Entrez Genepabpc1.S (a.k.a. XELAEV_18033915mg, pab1, pabp, pabp1, pabpc1, pabpc1-a, pabpc1-b, pabpc1.L, pabpc2)
-
Tag
/ Fusion Protein
- 6xHIS (C terminal on backbone)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
C064 was a gift from David Bartel (Addgene plasmid # 174353 ; http://n2t.net/addgene:174353 ; RRID:Addgene_174353) -
For your References section:
The molecular basis of coupling between poly(A)-tail length and translational efficiency. Xiang K, Bartel DP. Elife. 2021 Jul 2;10. pii: 66493. doi: 10.7554/eLife.66493. 10.7554/eLife.66493 PubMed 34213414