MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin)
(Plasmid
#174336)
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PurposeThe ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 174336 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMO70
- Backbone size w/o insert (bp) 6700
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman Orai1
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SpeciesH. sapiens (human)
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Insert Size (bp)2000
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GenBank IDNM_032790.3 NM_032790.3
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Entrez GeneORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)
- Promoter CMV
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Tag
/ Fusion Protein
- pHluorin inserted in the extracellular loop 2 of ORAI1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin) was a gift from Sonal Srikanth (Addgene plasmid # 174336 ; http://n2t.net/addgene:174336 ; RRID:Addgene_174336) -
For your References section:
NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca(2+) entry in T cells. Wu B, Woo JS, Vila P, Jew M, Leung J, Sun Z, Srikanth S, Gwack Y. Cell Rep. 2021 Aug 24;36(8):109603. doi: 10.1016/j.celrep.2021.109603. 10.1016/j.celrep.2021.109603 PubMed 34433025
