pEGFPC1-ORAI1-EC-HA
(Plasmid #174335)

• Purpose: encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA).
• Depositing Lab: Sonal Srikanth
• Publication: Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi: 10.1016/j.celrep.2021.109603.

Backbone

• Vector backbone: pEGFPN1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human Orai1
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• GenBank ID: NM_032790.3 NM_032790.3
• Entrez Gene: ORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP in the N-terminus and HA tag in the extracellular loop 2 of ORAI1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry