pN1mcherry-NKD2EFMUT
(Plasmid #174333)

• Purpose: encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.
• Depositing Lab: Sonal Srikanth
• Publication: Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi: 10.1016/j.celrep.2021.109603.

Backbone

• Vector backbone: pmCherryN1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Homo sapiens NKD inhibitor of WNT signaling pathway 2
• Alt name: NKD2
• Species: H. sapiens (human)
• Insert Size (bp): 1355
• Mutation: NKD2132DFD134>AFA (substitutions of D132 and D134 to alanine within the EF-hand loop)
• GenBank ID: NM_033120.4
• Entrez Gene: NKD2 (a.k.a. Naked2)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry tag (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry